Bio 12 (16-17) 29 Date Dec 14th
|
Last lessons Objective
|
Introduction to enzymes |
Evaluation |
| Today’s Objectives |
1. Inquiry based enzyme lab 2. Lab outline 3. Due Friday
|
|
| Topic
Number One |
In the last class we tested to find out how to use as spectrometer to select the actions of an enzyme. In this lab you will need to propose and carry out a test involving enzymes
|
|
| Topic
Number Two |
When writing up your lab, you need to do the following
Make sure to type up! Not hand written. Title: What were you testing or examinining
Purpose: State the purpose of your lab and provide a hypothesis and reason for the hypothesis. Cite a reference to show previous information about enzymes and the enzyme amylase.
Material Be very specific with this section, make sure to give precise measurement. Make sure to everything you used
Procedure (in point form) Starting from the beginning, make sure to outline everything you did. You may use this section to examine why experimental error occurred. Imagine that if you picked up your lab, you would know exactly each step of the lab. Assume nothing.
Data: In this section record both qualitative and quantitative values.
Results Make a graph of your data. Include a title, x and y coordinates and use either a line or bar graph.
Discussion and Summary To include: · Properties of the graph such as slope or if the graph is exponential and why · Discuss how your data and graph shows current understanding about enzymes and enzyme action. · You can even find a reference to compare your results with results found in your reference. · Discussion of possible experimental error.
Conclusion To include: · Hypothesis of lab verses results · Experimental error · Next variable to check
|
|
| Topic
Number Three |
Online References
Enzyme Lab http://www.und.nodak.edu/dept/jcarmich/101lab/lab6/lab6.html
Amylase Lab http://www.laney.edu/wp/cheli-fossum/files/2012/01/10-Enzymes.pdf
Amylase lab
Distinct questions to answer in your discussion · What is the difference between using Iodine verses Benedicts solution? · Which enzyme would be subject to denaturing and why?
|
|
| Text book Reference
|
Chapter 6 | |
| You tube Reference | Protein Denaturing Lab
https://www.youtube.com/watch?v=0EF7LZ4X8MU
Indicating sugar with iodine https://www.youtube.com/watch?v=cZyq4koUCNM
Indicators
|
|
| Take Home Message | A sailor noticed a sailboat with a feline on the end of boom. The boat was almost about to tip over. He pointed to it and said to his ship mates “Look…a cat a list!”
https://en.wikipedia.org/wiki/Glossary_of_nautical_terms |
No comments