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Bio 12 (16-17) L 25 Dec 8th

Bio 12 (16-17) L25                              Date: Dec 8 2017


Last lessons


Class Notes or Information

1.   Role of Enzymes

2.   Action of enzymes and changes due to ph, heat, acid and heavy metal





Today’s Objective 1)   Metabolic pathways

2)   Key reactions that can be monitored ( amylase and peroxidase)

3)   Planning to use spectrometer



Number One

Metabolic Pathways


We are on page 5 of our notes!

Metabolism is the sum of all chemical reactions within an organism.

Put simply, energy allows things to move.

Energy can be stored or used by the body.

Key players in the metabolic game of energy are ATP, Oxygen, ATP, Vitamins, Calcium and yes..Enzymes.


A cell can move, grow and reproduce. In each case you need energy.


Energy can be potential or kinetic


Potential is stored energy

Kinetic energy is linked to breaking down stored energy


Key terms: Anabolic (make) and Catabolic (break)


So simplify: I ate cereal and now I have energy.

a)   Glycolysis is the break down of sugar.

b)   By breaking down sugar, the body can convert products into ATP.

c)    By adding Hydrogen to Oxygen, the body has an efficient way to make ATP


To move you need energy, so does a cell.

In order, energy demands can be met by breaking down

a)   complex sugars to monosaccride, then to atp

b)   complex fats broken down to fatty acids can merge with sugar being metabolized.

c)    Complex proteins can be broken down and added to sugar metabolic pathway.


To break things down, you need energy.

You do not want to use more energy to break things down, than what you will get from catabolic pathways..

So we need enzymes!


See page 6 of notes


Khan explaination



Another video




Number Two

First of all a shout out for enzymes..

Bozeman Science



So how can we observe an enzyme reaction?


Note: Substrate plus enzyme will make product


If you change the amount of substrate, you need more enzyme.


If you have a functional enzyme it will make a product.

You can measure a variety of enzyme reactions by identifying the product.


Some easy enzyme reactions to monitor are:


Amylase reaction



Starch is a poly saccharide to break it down you can make into a disaccharide. What is the difference between amylase and maltase?


A story on amylase



Catalase Reaction



Peroxide (H2O2) bleaches your hair, cleans up a would but can affect your body. Catalase converts peroxide into water and oxygen!


Is there a metabolic relationship between amylase and catalase?



Number Three

If you change the structure of an enzyme, you will decrease the amount of product.

Some changes are permanent.

Some changes do not change the structure of the enzyme.


Lab with spectrometer and temperature




Sample amylase lab




Sample of a published amylase lab



Indicators and enzyme reaction products.


What does IKI bond to?

What does Benedicts solution bond to?




Text Reference Chapter 6, see chapter 6 practice quiz


Google hunt Mader Chapter quizzes






Class Notes References Chapter 6 in text  
Take Home message



How is meat tenderizer related to enzymes?
posted by Marc Bernard Carmichael in Biology 12,Biology 12 Lesson Outline and have No Comments

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