" The Big Picture!" by Mr C

1.   Role of Enzymes

2.   Action of enzymes and changes due to ph, heat, acid and heavy metal

2)   Key reactions that can be monitored ( amylase and peroxidase)

3)   Planning to use spectrometer

Number One

We are on page 5 of our notes!

Metabolism is the sum of all chemical reactions within an organism.

Put simply, energy allows things to move.

Energy can be stored or used by the body.

Key players in the metabolic game of energy are ATP, Oxygen, ATP, Vitamins, Calcium and yes..Enzymes.

A cell can move, grow and reproduce. In each case you need energy.

Energy can be potential or kinetic

Potential is stored energy

Kinetic energy is linked to breaking down stored energy

Key terms: Anabolic (make) and Catabolic (break)

So simplify: I ate cereal and now I have energy.

a)   Glycolysis is the break down of sugar.

b)   By breaking down sugar, the body can convert products into ATP.

c)    By adding Hydrogen to Oxygen, the body has an efficient way to make ATP

To move you need energy, so does a cell.

In order, energy demands can be met by breaking down

a)   complex sugars to monosaccride, then to atp

b)   complex fats broken down to fatty acids can merge with sugar being metabolized.

c)    Complex proteins can be broken down and added to sugar metabolic pathway.

To break things down, you need energy.

You do not want to use more energy to break things down, than what you will get from catabolic pathways..

So we need enzymes!

See page 6 of notes

Khan explaination

https://www.khanacademy.org/partner-content/stanford-medicine/growth-and-metabolism/v/basics-of-metabolism

Another video

https://www.youtube.com/watch?v=zVx9Qhj43E0

Number Two

Bozeman Science

https://www.youtube.com/watch?v=ok9esggzN18

So how can we observe an enzyme reaction?

Note: Substrate plus enzyme will make product

If you change the amount of substrate, you need more enzyme.

If you have a functional enzyme it will make a product.

You can measure a variety of enzyme reactions by identifying the product.

Some easy enzyme reactions to monitor are:

Amylase reaction

http://intro.bio.umb.edu/OLLM/111F98/pdfs/Amylase.pdf

Starch is a poly saccharide to break it down you can make into a disaccharide. What is the difference between amylase and maltase?

A story on amylase

https://www.youtube.com/watch?v=HTJy3lnPmL8

Catalase Reaction

https://www.scientificamerican.com/article/bring-science-home-liver-helping-enzymes/

Peroxide (H2O2) bleaches your hair, cleans up a would but can affect your body. Catalase converts peroxide into water and oxygen!

Is there a metabolic relationship between amylase and catalase?

Number Three

Some changes are permanent.

Some changes do not change the structure of the enzyme.

Lab with spectrometer and temperature

http://www.pierce.ctc.edu/staff/dblum/biology_160/list_and_laboratory_handouts/enzyme_spec2010.pdf

Sample amylase lab

http://www.und.nodak.edu/dept/jcarmich/101lab/lab6/lab6.html

http://www.laney.edu/wp/cheli-fossum/files/2012/01/10-Enzymes.pdf

Sample of a published amylase lab

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.617.5889&rep=rep1&type=pdf

Indicators and enzyme reaction products.

What does IKI bond to?

What does Benedicts solution bond to?

https://www.hccfl.edu/media/571427/3-indicator%20tests%20for%20important%20nutrients.pdf

http://www.mhhe.com/cgi-bin/webquiz.pl

http://www.mhhe.com/biosci/genbio/maderinquiry/quiz.html