" The Big Picture!" by Mr C

Last lessons Objective

Intro to bacteria

* Make sure to do vocab sheet for pop vocab quiz

1.   Sterile technique, Antiseptics and disinfectants

2.   Doing a gram stain

3.   Drawing known slide of bacteria verses yogurt

Number One

The sterile technique, antiseptics and disinfectants

Here are some great resources

Antiseptic,disinfectants and sterilization

http://study.com/academy/lesson/antiseptics-disinfectants-sterilization.html

Lister and antiseptic surgery (Great Scot!)

http://www.abpischools.org.uk/page/modules/infectiousdiseases_timeline/timeline5.cfm?coSiteNavigation_allTopic=1

Wound antiseptics and sterile techniques

https://meds.queensu.ca/central/assets/modules/basic_suturing/wound_antisepsis_and_sterile_technique.html

Focus question

With each technique what is being cleaned or killed?

How did sterile technique save lives?

Number Two

A “gram” stain is a means to identify if a bacteria has a cell wall or not.

We use two stain, one to stain the cell wall and the other to stain the cell membrane

Gram stains can also be used to identify pathogenic bacteria

Online Rf

http://www.uphs.upenn.edu/bugdrug/antibiotic_manual/Gram2.htm

Staining for gram positive and negative

http://www.microscope.com/education-center/how-to-guides/grams-stain/

Source for bacteria will be yogurt

You will make a slide, draw what you see and then research what bacteria are found in yogurt.

Number Three

Next class we will design an experiment to test a gram positive and gram negative bacteria.

Here are some references to discuss the biology of gram positive and gram negative bacteria

http://www.diffen.com/difference/Gram-negative_Bacteria_vs_Gram-positive_Bacteria

Sample Lab

http://www.hometrainingtools.com/a/gram-stain-antibiotics-project

True Bacteria Notes on Blog

http://www.ucmp.berkeley.edu/bacteria/bacteria.html

Microbiology on line (from the UK)

http://www.microbiologyonline.org.uk/about-microbiology/introducing-microbes/bacteria

Micro facts that may make you feel dirty

https://www.youtube.com/watch?v=Lg-1Vv4-58M

Bacteria and virus

https://www.youtube.com/watch?v=3xRttWuf3wQ

·      What would you spray on your cell phone to kill off bacteria? An antiseptic or a disinfectant..why?

·      Why do we put the inoculation loop through the Bunsen flame?

·      When you heat up glass..it gets hot…very hot.

·      Why do basic solutions such as windex make great killers of bacteria?