" The Big Picture!" by Mr C

Last lessons Objective

Introduction to enzymes

1.   Inquiry based enzyme lab

2.   Lab quiz on Thursday lunch or Friday

3.   Exam on Wednesday (DNA,RNA and Enzymes)

Number One

You are provided with three protein powders, labeled as A, B, and C.

You are told that two of the powders are enzyme diastase, amylase and the third powder is a non-enzyme protein.

In two stages, you need to design a lab to decide which is the non-enzyme and then, which is the amylase and diastase.

You also need to have a control.

The substrate you are using is starch.

Your indicator is Benedicts solution that turns yellow when there are monosaccrides.

You should write up the lab using the outline in section two

Number Two

Title

Purpose

Material

Procedure (in point form)

Results Table

Discussion

To include:

·      Identify each powder and explain why

·      Discuss evidence and facts about denaturing due to   temperature

·      Discussion of possible experimental error

·      Discussion about the activity of amylase verse diastase

Conclusion

To include:

·      Hypothesis of lab verses results

·      Experimental error

·      Next variable to check

Number Three

Enzyme Lab

http://www.und.nodak.edu/dept/jcarmich/101lab/lab6/lab6.html

Amylase Lab

http://www.laney.edu/wp/cheli-fossum/files/2012/01/10-Enzymes.pdf

Amylase lab

https://www.pearsonschoolsandfecolleges.co.uk/Secondary/Science/Software/ModularScienceForAQA/Samples/Samplematerial/mod_sci_aqa_trp.pdf

Distinct questions to answer in your discussion

·      What is the difference between using Iodine verses Benedicts solution?

·      Which enzyme would be subject to denaturing and why?